We are looking for a highly motivated Research Assistant to join our group to investigate how the cardiac vasculature system develops and how it supports the regeneration of heart tissue after injury. The successful applicant will join Michael Harrison’s lab in Cardiovascular Research Institute (https://cvri.weill.cornell.edu/) at Weill Cornell Medicine in New York.
The lab is focused on both components of the cardiac vasculature, the coronary vessels that supply the heart with blood and the lymphatic vessels that remove fluid and debris from cardiac tissue. We primarily study zebrafish which have enhanced cardiac repair compared to mice and humans. We want to identify dormant pathways and processes that can be utilized to accelerate regeneration in patients.
We utilize novel imaging techniques and zebrafish genetic models to dissect the cues driving vascularization and vascular mediation of heart regeneration.
Successful candidates will primarily help with:
- Management of the zebrafish colony including breeding, screening transgenic embryos, tracking fish numbers and tank densities. Work with the institute’s animal husbandry team that provide feeding and maintain the aquarium system.
- Genotyping (PCR and electrophoresis)
- Basic bench techniques including preparing solutions, sterile technique.
- Histology – sectioning and staining tissues
- Making transgenic and mutant zebrafish – including molecular biology and genome editing techniques, embryo microinjection.
- Imaging – whole mount, live, confocal and tissue sections
- Sequencing – preparing and analysis of bulk and single-cell sequencing samples
- Assist in basic laboratory tasks and ordering supplies
- Ensuring that safe laboratory practices are followed, including the use and disposal of chemicals and hazardous materials.
Experience with molecular biology, imaging, or zebrafish handling highly desired.
Bachelor's or Master's Degree in biology or related field.
Minimum of 1 year of wet lab bench (academic or industry) experience preferred.
At least a two-year commitment preferred due to a substantial training effort associated with the position.
Knowledge, Skills and Abilities
Demonstrated organizational skills and ability to pay close attention to detail.
Motivation to learn and grow in scientific expertise.
Ability to manage work with minimal supervision.
Ability to trouble-shoot technical issues.
Strong communication and presentation skills.
Ability to work on multiple projects simultaneously.
Ability to plan and prioritize work while responding flexibly to rapidly changing priorities.
Ability to work flexible hours.
This position may require standing or sitting for long periods of time, including in a warm aquarium environment.